Dmem f12 composition4/6/2024 These two monolayers were comparatively evaluated regarding reliability and stability by morphology, transepithelial electrical resistance (TEER), alkaline phosphatase (AKPase) activity and transport experiments. Two Caco-2 cell monolayers cultured in both media were compared herein in order to underlay the standardization of this model. Dulbecco's Modified Eagle's Medium (DMEM) and Minimum Essential Medium (MEM) have been used alternatively in the development of this model, although they are different in composition which may affect the differentiation and junction formation of the Caco-2 cell monolayer. The Caco-2 cell monolayer model is widely used in drug absorption studies.
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